Isolation of human DNA clones from bacteriophage lambda libraries that identify variable number tandem repeat loci

Date
1989
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Gene mapping is a very active area of research in human genetics. This effort is still in its infancy, with less than 2% of the expected gene complement having been mapped to chromosomes. Recent advances in DNA technology that permit the detection of DNA sequence variations, should provide the large number of highly polymorphic loci necessary to map the total human genome with high resolution. Isolation of new DNA clones that recognize highly polymorphic loci constitutes an important component of the gene mapping process. Based on its size, chromosome 9 is relatively underrepresented in terms of the available number of DNA markers. This fact, coupled with the tentative localization of the gene responsible for tuberous sclerosis, provided the incentive to attempt to isolate new chromosome 9 DNA markers. Library screening methods using oligonucleotide probes and competitive hybridization similar to those used for cosmid libraries were successfully employed to isolate a highly polymorphic marker from an unamplif ied human total genomic lambda library. Though this study failed in its attempt to isolate new highly polymorphic chromosome 9 markers from two chromosome 9-enriched lambda libraries, these studies reiterated the importance of constructing and maintaining lambda libraries with recombination deficient hosts which do not select against the repetitive sequences associated with these loci. This study demonstrates that unamplified lambda libraries can be used as a source of DNA clones that recognize variable number tandem repeat loci and defines parameters for the selection of appropriate libraries and efficient isolation of new highly polymorphic DNA markers.
Description
Bibliography: p. 90-98.
Keywords
Citation
Kraus, S. L. (1989). Isolation of human DNA clones from bacteriophage lambda libraries that identify variable number tandem repeat loci (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/20938
Collections