Please use this identifier to cite or link to this item: http://hdl.handle.net/1880/51152
Title: Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani
Authors: McNeil, Bonnie A
Simon, Dawn M
Zimmerly, Steven
Issue Date: Oct-2013
Publisher: Oxford Journals
Citation: McNeil, B.A., Simon, D.M., Zimmerly, S. (2014) Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani. Nucleic Acids Research 42:1959-1969. doi: 10.1093/nar/gkt1053
Abstract: Group II introns are ribozymes and retroelements found in bacteria, and are thought to have been the ancestors of nuclear pre-mRNA introns. Whereas nuclear introns undergo prolific alternative splicing in some species, group II introns are not known to carry out equivalent reactions. Here we report a group II intron in the human pathogen Clostridium tetani, which undergoes four alternative splicing reactions in vivo. Together with unspliced transcript, five mRNAs are produced, each encoding a distinct surface layer protein isoform. Correct fusion of exon reading frames requires a shifted 5' splice site located 8 nt upstream of the canonical boundary motif. The shifted junction is accomplished by an altered IBS1-EBS1 pairing between the intron and 5' exon. Growth of C. tetani under a variety of conditions did not result in large changes in alternative splicing levels, raising the possibility that alternative splicing is constitutive. This work demonstrates a novel type of gene organization and regulation in bacteria, and provides an additional parallel between group II and nuclear pre-mRNA introns.
URI: http://hdl.handle.net/1880/51152
Appears in Collections:Zimmerly, Steven

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