Isolation of human DNA clones from bacteriophage lambda libraries that identify variable number tandem repeat loci

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dc.contributor.advisorBech-Hansen, N. Torben
dc.contributor.authorKraus, Steven L.
dc.date.accessioned2005-07-21T19:37:47Z
dc.date.available2005-07-21T19:37:47Z
dc.date.issued1989
dc.descriptionBibliography: p. 90-98.en
dc.description.abstractGene mapping is a very active area of research in human genetics. This effort is still in its infancy, with less than 2% of the expected gene complement having been mapped to chromosomes. Recent advances in DNA technology that permit the detection of DNA sequence variations, should provide the large number of highly polymorphic loci necessary to map the total human genome with high resolution. Isolation of new DNA clones that recognize highly polymorphic loci constitutes an important component of the gene mapping process. Based on its size, chromosome 9 is relatively underrepresented in terms of the available number of DNA markers. This fact, coupled with the tentative localization of the gene responsible for tuberous sclerosis, provided the incentive to attempt to isolate new chromosome 9 DNA markers. Library screening methods using oligonucleotide probes and competitive hybridization similar to those used for cosmid libraries were successfully employed to isolate a highly polymorphic marker from an unamplif ied human total genomic lambda library. Though this study failed in its attempt to isolate new highly polymorphic chromosome 9 markers from two chromosome 9-enriched lambda libraries, these studies reiterated the importance of constructing and maintaining lambda libraries with recombination deficient hosts which do not select against the repetitive sequences associated with these loci. This study demonstrates that unamplified lambda libraries can be used as a source of DNA clones that recognize variable number tandem repeat loci and defines parameters for the selection of appropriate libraries and efficient isolation of new highly polymorphic DNA markers.
dc.format.extentxvii, 98 leaves : ill. ; 30 cm.en
dc.identifier.citationKraus, S. L. (1989). Isolation of human DNA clones from bacteriophage lambda libraries that identify variable number tandem repeat loci (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/20938en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/20938
dc.identifier.isbn0315542594en
dc.identifier.lccQH 445.2 K73 1989en
dc.identifier.urihttp://hdl.handle.net/1880/21629
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject.lccQH 445.2 K73 1989en
dc.subject.lcshChromosome mapping
dc.subject.lcshMolecular cloning
dc.titleIsolation of human DNA clones from bacteriophage lambda libraries that identify variable number tandem repeat loci
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 703 520541625
ucalgary.thesis.notesoffsiteen
ucalgary.thesis.uarcreleaseyen
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