In vivo Sarcomere Lengths and Sarcomere Elongations Are Not Uniform across an Intact Muscle

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dc.contributor.authorHerzog, Walter
dc.contributor.authorMoo, Eng Kuan
dc.contributor.authorFortuna, Rafael
dc.contributor.authorSibole, Scott C.
dc.contributor.authorAbusara, Ziad
dc.date.accessioned2016-06-21T19:58:12Z
dc.date.available2016-06-21T19:58:12Z
dc.date.issued2016-05
dc.description.abstractSarcomere lengths have been a crucial outcome measure for understanding and explaining basic muscle properties and muscle function. Sarcomere lengths for a given muscle are typically measured at a single spot, often in the mid-belly of the muscle, and at a given muscle length. It is then assumed implicitly that the sarcomere length measured at this single spot represents the sarcomere lengths at other locations within the muscle, and force-length, force-velocity, and power-velocity properties of muscles are often implied based on these single sarcomere length measurements. Although, intuitively appealing, this assumption is yet to be supported by systematic evidence. The objective of this study was to measure sarcomere lengths at defined locations along and across an intact muscle, at different muscle lengths. Using second harmonic generation (SHG) imaging technique, sarcomere patterns in passive mouse tibialis anterior (TA) were imaged in a non-contact manner at five selected locations (“proximal,” “distal,” “middle,” “medial,” and “lateral” TA sites) and at three different lengths encompassing the anatomical range of motion of the TA. We showed that sarcomere lengths varied substantially within small regions of the muscle and also for different sites across the entire TA. Also, sarcomere elongations with muscle lengthening were non-uniform across the muscle, with the highest sarcomere stretches occurring near the myotendinous junction. We conclude that muscle mechanics derived from sarcomere length measured from a small region of a muscle may not well-represent the sarcomere length and associated functional properties of the entire muscle.en_US
dc.description.refereedYesen_US
dc.identifier.citationMoo, E. K., Fortuna, R., Sibole, S. C., Abusara, Z., & Herzog, W. (2016). In vivo sarcomere lengths and sarcomere elongations are not uniform across an intact muscle. Frontiers in physiology, 7.en_US
dc.identifier.doi10.3389/fphys.2016.00187
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/34241
dc.identifier.urihttp://hdl.handle.net/1880/51421
dc.language.isoenen_US
dc.publisherFrontiers in Physiologyen_US
dc.publisher.departmentHuman Performance Laboratoryen_US
dc.publisher.facultyKinesiologyen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.subjectsarcomere lengthen_US
dc.subjectnon-uniformityen_US
dc.subjectsecond harmonic generation imagingen_US
dc.subjectmyosin banden_US
dc.subjectin vivoen_US
dc.subjectpassive lentheningen_US
dc.titleIn vivo Sarcomere Lengths and Sarcomere Elongations Are Not Uniform across an Intact Muscleen_US
dc.typejournal article
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