PCR/DNA analysis of archaeological faunal remains from Rocky Mountain House and Morleyville (1834-1861, 1875-1896), Alberta, Canada

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dc.contributor.advisorCeri, Howard
dc.contributor.authorPelly, Lorine P.
dc.date.accessioned2005-08-16T17:17:56Z
dc.date.available2005-08-16T17:17:56Z
dc.date.issued2004
dc.descriptionBibliography: p. 105-114en
dc.descriptionSome pages are in colour.en
dc.description.abstractTwo settlements in south central Alberta, Rocky Mountain House, a fur trade post, and Morleyville, a Methodist mission, were occupied contemporaneously and situated in close proximity in the same geographic region. Both settlements contained substantial quantities of fragmentary bone making it difficult to specifically identify the species of origin. Ancient DNA analysis was employed to determine the species of origin of a significant number of bones from both sites. It was believed that ancient DNA analysis would help clarify faunal utilization patterns at Rocky Mountain House and Morleyville. Primers were designed to amplify a 124 base pair fragment of the cytochrome b gene in ruminants. Elk, moose, bison, cow and caribou sequences were recovered from Rocky Mountain House, a fur trade post. Elk was most highly represented at approximately 65% of the sequences recovered. This contradicts the conventional wisdom that bison were the most common food source here. Cow, elk, bison, pig and mule deer sequences were recovered from Morleyville. Ancient DNA analysis allowed the separation of cow and bison remains at Morleyville, which previously had not been possible. Since unidentified bones at Rocky Mountain House were reasonably abundant, this presented an opportunity to examine predictors of DNA survival. It was expected that an association would exist between the ability to amplify DNA and the state of preservation of the bone. It was not possible to confirm this expectation.en
dc.format.extentviii, 114 leaves : ill. ; 30 cm.en
dc.identifier.citationPelly, L. P. (2004). PCR/DNA analysis of archaeological faunal remains from Rocky Mountain House and Morleyville (1834-1861, 1875-1896), Alberta, Canada (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/14789en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/14789
dc.identifier.isbn0612976106en
dc.identifier.lccAC1 .T484 2004 P45en
dc.identifier.urihttp://hdl.handle.net/1880/41877
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titlePCR/DNA analysis of archaeological faunal remains from Rocky Mountain House and Morleyville (1834-1861, 1875-1896), Alberta, Canada
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1528 520492045
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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