Multi-pathway control of the proliferation versus meiotic development decision in the Caenorhabditis elegans germline

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dc.contributor.authorHansen, Dave D.en
dc.contributor.authorHubbard, E. Jane Alberten
dc.contributor.authorSchedl, Timen
dc.contributor.departmentBiological Sciencesen
dc.contributor.facultyFaculty of Scienceen
dc.contributor.institutionUniversity of Calgaryen
dc.date.accessioned2006-08-15T16:23:18Z
dc.date.available2006-08-15T16:23:18Z
dc.date.issued2004
dc.description2004 Elsevier Inc. All rights reserved.en
dc.description.abstractAn important event in the development of the germline is the initiation of meiotic development. In Caenorhabditis elegans, the conserved GLP-1/Notch signaling pathway regulates the proliferative versus meiotic entry decision, at least in part, by spatially inhibiting genes in the gld-1 and gld-2 parallel pathways, which are proposed to either inhibit proliferation and/or promote meiotic development. Mutations that cause constitutive activation of the GLP-1 pathway, or inactivation of both the gld-1 and gld-2 parallel pathways, result in a tumorous germline in which all cells are thought to be proliferative. Here, to analyze proliferation and meiotic entry in wild-type and mutant tumorous germlines, we use anti-REC-8 and anti-HIM-3 specific antibodies as markers, which under our fixation conditions, stain proliferative and meiotic cells, respectively. Using these makers in wild-type animals, we find that the border of the switch from proliferation to meiotic entry is staggered in late-larval and adult germlines. In wild-type adults, the switch occurs between 19 and 26 cell diameters from the distal end, on average. Our analysis of mutants reveals that tumorous germlines that form when GLP-1 is constitutively active are completely proliferative, while tumors due to inactivation of the gld-1 and gld-2 pathways show evidence of meiotic entry. Genetic and time course studies suggest that a third pathway may exist, parallel to the GLD-1 and GLD-2 pathways, that promotes meiotic development.en
dc.description.refereedyesen
dc.format.extent918865 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationDave Hansen, E. Jane Albert Hubbard,Tim Schedl "Multi-pathway control of the proliferation versus meiotic development decision in the Caenorhabditis elegans germline" Developmental Biology 268 (2004) 342–357en
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/35125
dc.identifier.issn0012-1606
dc.identifier.urihttp://hdl.handle.net/1880/43437
dc.language.isoenen
dc.publisherElsevieren
dc.publisher.urlhttp://www.elsevier.com/wps/find/journaldescription.cws_home/622816/description#description
dc.subjectBiologyen
dc.subject.otherGermline developmenten
dc.subject.otherProliferationen
dc.subject.otherMeiotic entryen
dc.subject.otherGermline tumoren
dc.subject.otherGLP-1/Notchen
dc.subject.othergld-1en
dc.subject.othergld-2en
dc.subject.othernos-3en
dc.titleMulti-pathway control of the proliferation versus meiotic development decision in the Caenorhabditis elegans germlineen
dc.typejournal article
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