Retention Characteristics of pH Tunable Water Stationary Phase in Supercritical Fluid Chromatography

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dc.contributor.authorThurbide, Kevin
dc.contributor.authorScott, Andrea
dc.date.accessioned2017-01-24T01:01:50Z
dc.date.available2017-01-24T01:01:50Z
dc.date.issued2016-12-19
dc.description.abstractThe retention characteristics of a novel pH tunable water stationary phase are presented. The method utilizes a change in mobile phase from N2 to CO2 to acidify the water phase in situ and control the ionization and elution of organic acids. With N2 present the phase pH > 5.4 and the acids are ionized and strongly retained. Conversely, with CO2 present the pH < 3.8 and the acids are neutralized and can elute. This effect is reasonably independent of time. For example, at 80°C hexanoic acid readily elutes from a 10 m column after switching to CO2 at any point over a 1 h period. Beyond this, however, some broadening and peak erosion is noted. Acids are also retained on 10 and 2 m columns similarly, since their elution primarily depends upon the change in stationary phase pH. Altering the CO2 solubility in the water phase alone (i.e., through changing system temperature and pressure without using N2) also produces similar changes in stationary phase acidity. However, this approach yields greater system noise and instability. The N2/CO2 switching mode is used to analyze organic acids in various samples and is found to provide high selectivity for them over other matrix components. Therefore, this approach can potentially simplify the analysis of such acids in complex samples.en_US
dc.description.refereedYesen_US
dc.identifier.citationJ Chromatogr Sci (2016) 55 (1): 82-89.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/35417
dc.identifier.urihttp://hdl.handle.net/1880/51794
dc.language.isoenen_US
dc.publisherJournal of Chromatographic Scienceen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.urlhttps://academic.oup.com/chromsci/article-abstract/55/1/82/2527510/Retention-Characteristics-of-a-pH-Tunable-Water?redirectedFrom=fulltexten_US
dc.titleRetention Characteristics of pH Tunable Water Stationary Phase in Supercritical Fluid Chromatographyen_US
dc.typejournal article
thesis.degree.disciplineChemistryen_US
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