Browsing by Author "Banoei, Mohammad Mehdi"

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    Metabolomics Study of the Diagnosis and Prognosis of Severe Traumatic Brain Injury (sTBI)
    (2020-11-30) Banoei, Mohammad Mehdi; Winston, Brent W; Habibi, Hamid R; Lewis, Ian A; Couillard, Phillippe; Debert, Chantel T; Fraser, Douglas D;
    Traumatic brain injury (TBI) is defined as neurologic injury resulting from an external mechanical force, which is associated with long-term neurological and cognitive disability and affects individuals of all ages, ethnicities, and socioeconomic characteristics. TBI severe enough to cause hospitalization occurs in over 10 million people each year worldwide. TBI is the most common cause of death and long-term disability in children and young adults in developed countries. Currently, clinical assessment and neuroimaging (e.g. CT and MRI) are the most reliable techniques used for the diagnosis and prognosis of TBI. Unfortunately, this approach has considerable shortcomings when considering sensitivity and specificity of TBI diagnosis and prognosis. Disease stratification and the prediction of outcomes and are key problems for the management of severe TBI (sTBI).This study showed that metabolomics can be applied for the diagnosis and prognosis of sTBI using nuclear magnetic resonance (NMR) spectroscopy and direct infusion tandem mass spectrometry (DI-MS/MS). The results are promising for the diagnose sTBI when compared to orthopedic injury (OI) controls and for the prognostication sTBI outcomes at 3, 6, and 12-months post-injury particularly in predicting poor outcomes from the good outcome. We also investigated the metabolomics on animal models for sTBI. Using the cortical controlled impact (CCI) mouse model of sTBI demonstrated a difference in metabolic profiles of CCI and CCI mice receiving a plastic CAP to cover the skull in the craniotome area (CCI+CAP) mice when compared to sham controls. Our result revealed that the highest degree of metabolic alterations occurs at 8 hours post-injury and that the CCI+CAP mice have a prolonged brain injury compared to CCI mice (assessed at 7 days post-injury).
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    Plasma Metabolomics of Community Acquired Pneumonia (CAP) For Prognosis of Patients at Highest Risk of Mortality
    (2015-07-13) Banoei, Mohammad Mehdi; Winston, Brent
    Community acquired pneumonia (CAP) is an acute disease with clinical features of lower respiratory tract infections. Early and accurate prognoses, and effective treatment, where possible, in the management of CAP are essential to decrease mortality. Metabolomic analysis is defined as the comprehensive study of low molecular-weight metabolites present in biological samples that enable the qualitative and/or quantitative measurement and analysis of multiple metabolites We showed the feasibility of metabolomics using nuclear magnetic resonance (NMR) spectroscopy, gas chromatography mass spectrometry (GC-MS) and direct infusion tandem mass spectrometry (DIMS/MS) as most common analytical platforms in association with multivariate statistical analysis to separate non-survivors and survivor of bacteria CAP and H1N1 pneumonia patients for prognosis of mortality. In conclusion, this study may not only be instrumental in predicting the mortality of CAP patients within 24 hours of hospitalization, but may also allow for the rapid identification of the infective organism in CAP.