Tendon and ligament explants are utilized in ACL reconstruction. After ACL reconstructive surgery, or ACL injury, there is an up-regulation of inflammation, which has been implicated in causing cellular death. The objective of this study is to examine the effect of cytokines on cells and tissue of ligaments and tendons. The mRNA expression of inflammatory and apoptotic markers were measured in primary cells after treatment with cytokines and cell death was measured with fluorescent dyes. Next ACL and PT explants were analyzed for mRNA expression. Furthermore, Annexin V and DNA laddering techniques were used to evaluate for apoptosis. Apoptosis does not seem to be a direct effect of cytokine treatment. In almost all treatment groups, SNP was the only reagent that caused significant amounts of apoptosis. Results showed evidence of a connection between IL1B, iNOS, and SNP. Additionally, tissues with more blade cuts had significantly higher amounts of cell death.