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Timing recombinant prion protein conversion as a measure of prion activity in chronic wasting disease

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Advisor
Czub, Markus
Author
Gray, John Geoffrey
Accessioned
2014-05-23T22:38:28Z
Available
2014-11-17T08:00:27Z
Issued
2014-05-23
Submitted
2014
Other
Prion
Chronic Wasting Disease
Detection
Elk
In vitro PrP conversion
RePLICA
Amyloid
Fluorescence
Subject
Biology--Molecular
Veterinary Science
Type
Thesis
Metadata
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Abstract
Chronic Wasting Disease (CWD) is a fatal neurological disease affecting cervids caused by prions. Infected cervids shed the CWD prion in bodily fluids and excrement, contaminating the environment and creating an agricultural and ecological calamity. Preclinical antemortem CWD testing method is demanded by CWD risk management programs. In vitro PrP-conversion assays have been developed as potential tools for such an approach with increasing sensitivity for prion detection. However, no method has been routinely employed thus far. Timing recombinant-PrP conversion into amyloid fibrils, seeded by elk CWD prion, as a diagnostic method is presented herein. The assay, termed “RePLICA”, is at least as sensitive for detecting elk CWD in brain tissues as Tg(CerPrP-M132)1536+/- and Tg(CerPrP-E226)5037+/- mouse bioassay models. The assay performs within a period of 35 hours, is consistently reproducible, and functions on elk brain and tonsil tissues. There are indications RePLICA has the potential to titre CWD infectivity.
Corporate
University of Calgary
Faculty
Graduate Studies
Doi
http://dx.doi.org/10.5072/PRISM/28457
Uri
http://hdl.handle.net/11023/1550
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