Detection of Antibodies Against the Open Reading Frame Three Protein of Porcine Circovirus 2 in Pigs
Date
2014-07-10
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Abstract
The swine pathogen porcine circovirus 2 (PCV2) is the primary etiological agent of a syndrome that is damaging to the swine industry. The PCV2 genome contains three major open reading frames (ORFs) and ORF3 appears to play a role in PCV2-related pathology and dissemination. No data have been published confirming that PCV2ORF3 protein is expressed in PCV2-infected swine. Here it is hypothesized that αPCV2ORF3 antibodies are present in the sera of most adult swine. Swine sera were screened for αPCV2ORF3 antibodies, the presence of which would imply PCV2ORF3 expression. About 90% of the tested sera from weaned swine appear to have at least some reactivity to rPCV2ORF3, implying that PCV2ORF3 is expressed in vivo. Serum reactivity to PCV2ORF3 did not correlate with PCV2 viral load or vaccination status. These results do not indicate or rule out any particular biological role for PCV2ORF3.
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Veterinary Science, Virology, Biochemistry
Citation
Hawkes, A. (2014). Detection of Antibodies Against the Open Reading Frame Three Protein of Porcine Circovirus 2 in Pigs (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25599