The Surprising Photophysics of mOrange
Date
2014-09-15
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Abstract
It is of little doubt that the discovery of fluorescent proteins (FPs) has dramatically advanced the fields of biology, medicine and chemistry. However, the inevitable loss of fluorescence through prolonged irradiation in a process known as photobleaching, is still not fully understood. Thus, through the investigation of one of the most well known photobleaching sensitive proteins, mOrange, further light has been shed on this topic. Unique to this investigation has been the photophysical approaches used in the elucidation of the loss of fluorescence kinetics, where the use of high-power light sources have revealed the contributions of the improperly folded green immature mOrange. Additionally, this green immature mOrange has shown to have a distinct link to the production of a red fluorescent by-product. Finally, it has been shown that the excitation of mOrange has the ability to produce modest amount of singlet oxygen (1O2), implicating 1O2 as a cause of photobleaching.
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Chemistry--Physical
Citation
Pearson, D. (2014). The Surprising Photophysics of mOrange (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28394