The Evaluation of MS4A4A and MS4A8B Expression in Hematopoietic Cells
Date
2014-09-23
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
MS4A is a family of proteins with four membrane spanning regions. Currently, among the 15+ human MS4A family members, most remain uncharacterized. A notable exception is MS4A1 (CD20) which displays restricted expression on the surface of B-lymphocytes and forms the immunotherapeutic target of Rituximab. Given the importance of MS4A1 as an immunotherapeutic target our goal was to search for additional members of the MS4A family that may form potential immunotherapeutic targets. Here, I describe the evaluation of I) MS4A8B and MS4A4A expression in select hematopoietic cell subsets, using monoclonal antibodies that were generated against extracellular epitopes, and II) the investigation of whether monoclonal antibodies directed against MS4A4A can induce Fc-receptor independent effects characteristic of immunotherapeutic antibodies such as cellular apoptosis. We found that MS4A8B is not expressed in any of the hematopoietic cell subsets evaluated but is present in the NCI-H69 small cell lung carcinoma cell line. However, we identified that MS4A4A is expressed in select hematopoietic cell subsets. Specifically, herein I report that MS4A4A is expressed in peripheral blood monocytes and monocyte derived M2 macrophages but not resting peripheral blood granulocytes, lymphocytes, or M1 macrophages. I also report that monoclonal antibodies directed against MS4A4A were not capable of inducing cellular apoptosis or influencing select cellular functions in the cells evaluated.
Description
Keywords
Biology, Biology--Molecular, Immunology
Citation
Puri, M. (2014). The Evaluation of MS4A4A and MS4A8B Expression in Hematopoietic Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28136