Monitoring changes in tissue oxygenation and blood volume is an important aspect of understanding the mechanisms of many diseases and creating corresponding therapeutic interventions. Spectroscopic imaging is adopted in my research to provide a non-contact recording method. Traditionally, such imaging is performed in anesthetized and restrained animals using benchtop instrumentation or miniaturized systems connecting to benchtop instruments through optical fibers or electrical cables, which limits the design and scope of studies. To create a miniaturized imaging system that realizes stand-alone operation on awake and freely-moving rats, I designed and implemented an imaging system, consisting of a miniaturized optical system, a high-performance complementary metal oxide semiconductor (CMOS) image sensor and a storage circuit. The system was electrically and optically characterized and proved to be capable of imaging reflectance changes related to tissue hemodynamics in response to deep brain stimulation (DBS).