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Serum-free Co-expansion of Mesenchymal Stem Cells and Chondrocytes as Aggregates in Suspension Bioreactors

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ucalgary_2014_khurshid_madiha.pdf (6.127Mb)
Advisor
Sen, Arindom
Adesida, Adetola
Author
Khurshid, Madiha
Accessioned
2014-12-15T23:33:08Z
Embargolift
2016-12-14T23:33:08Z
Issued
2014-12-15
Submitted
2014
Other
tissue engineering
bioprocess development
suspension bioreactors
cell co-culture
cell aggregates
serum-free medium
mesenchymal stem cells
chondrocytes
bioprocess engineering
Subject
Engineering--Biomedical
Engineering--Chemical
Type
Thesis
Metadata
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Abstract
Defects in articular cartilage can develop into osteoarthritis, a condition that affects 10% of Canadians. A clinical method of cartilage repair involves the implantation of articular chondrocytes (ACs) into the defect. Mesenchymal stem cells (MSCs) can also be used in this application, after differentiation into chondrocytes. It is difficult to expand either of these cell populations without decreasing the quality of cartilage tissues produced. However, the co-culture of ACs and MSCs can produce enhanced cartilage tissues in tissue culture flasks (T-flasks). The scale-up of this co-expansion under serum-free conditions is necessary for clinical translation. A serum-free medium (SFM) called PPRF-msc6 was shown to support cell co-expansion. Additionally, these cells were successfully co-expanded in scalable suspension bioreactors as three-dimensional aggregates, achieving cell densities of 95,000 cells/mL and producing more matrix than in T-flasks. Suspension bioreactors can co-expand ACs and MSCs as aggregates using SFM for potential utility in cartilage repair strategies.
Embargoterms
2 years
Corporate
University of Calgary
Faculty
Graduate Studies
Doi
http://dx.doi.org/10.11575/PRISM/27759
Uri
http://hdl.handle.net/11023/1963
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