Quantification of HIV-1 Proviral DNA Forms in Gut Tissues Using Real-Time PCR
Date
2015-09-29
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Previous studies have shown that infection of gut tissues (esophagus-stomach-duodenum-colon) by HIV is compartmentalized with increased HIV-1 diversity and selection for drug resistance in the colon. We looked at the kinetics of HIV-1 in the gut tissues obtained from infected patients receiving ART (i.e AZT or ddI). We established real- time PCR protocols to detect HIV-1 proviral DNA forms. The total HIV-1 proviral DNA was the most frequent form found in the gut tissues. 2-LTR proviral DNA was also detected but in low levels which might indicate low viral replication. HIV-1 latent infection was also detected in the gut tissues as represented by the integrated proviral DNA levels. The levels detected of the proviral DNA forms varied between gut tissues, visits and patients, and no difference or correlation were found for patients receiving AZT or ddI.
Description
Keywords
Microbiology
Citation
Bahafzallah, L. (2015). Quantification of HIV-1 Proviral DNA Forms in Gut Tissues Using Real-Time PCR (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27495