Establishing a Fluorescence in situ Hybridization Approach for Subcellular mRNA Localization in Arabidopsis thaliana

Date
2016-01-27
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Abstract
Post-transcriptional regulation of gene expression is an important cellular process that occurs in eukaryotes during development and in response to environmental cues. Subcellular mRNA localization is one type of post-transcriptional regulation that serves to control translation in a spatial and temporal manner. In higher plants, reports on subcellular mRNA localization are limited and the frequency of this mechanism in different cell types is unknown. The aim of this thesis was to optimize a fluorescence whole mount in situ hybridization approach in Arabidopsis thaliana with the goal of initiating a large scale screen for examples of subcellular mRNA localization in plants. Multiple visualization techniques were employed, including fluorescent tyramide signal amplification and single mRNA fluorescence approaches. The subcellular localization pattern of three mRNAs is reported here in seedling cotyledon and young leaf cells that were visualized using the optimized fluorescence whole mount in situ hybridization approach.
Description
Keywords
Biology--Cell, Biology--Molecular
Citation
Halbauer, J. (2016). Establishing a Fluorescence in situ Hybridization Approach for Subcellular mRNA Localization in Arabidopsis thaliana (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25953