Expansion of Skin-Derived Precursor Cells (SKPs) in Stirred Suspension Bioreactors
Abstract
Skin-derived precursor cells (SKPs) have potential therapeutic applications for dermal regeneration in patients who have undergone split-thickness skin graft (STSG) surgeries due to severe burns. While these cells are traditionally grown in vitro in static t-flasks, a more standardized, well-controlled culture environment is desirable so that sufficient numbers of cells can be produced for clinical applications. This study outlines a bioprocess which could be used to expand SKPs, and establishes a baseline of how these cells grow in static and bioreactor conditions. Using a 9% total body surface area burn as the baseline, it was determined that a fold expansion of approximately 45 to 270 would be required for a 10 cm² piece of donor skin, assuming all cells isolated proliferate and are of therapeutic benefit. While this is not feasible based on current expansion achieved in bioreactors, this study provides useful information moving forward towards more advanced bioprocess development.
Description
Keywords
Biology--Cell, Medicine and Surgery, Engineering--Biomedical, Engineering--Chemical
Citation
Boon, K. (2016). Expansion of Skin-Derived Precursor Cells (SKPs) in Stirred Suspension Bioreactors (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26042