Effect of Indomethacin on Kidney Development in Postnatal Period in Rats
Abstract
Nephrogenesis in the human ends by the 36th week of gestation. In premature infants,
nephrogenesis is assumed to continue after birth. Indomethacin (NSAID) is commonly used to
treat patent ductus arteriosus (PDA) in premature infants. We hypothesized that administration of
indomethacin in the newborn rats would cause a reduction in glomerular number. Four dosegroups
of rats (n=7 in each) were injected intraperitoneally with three different doses (0.1, 0.5, 1
mg/kg) of indomethacin or a saline control for 11 days after birth. At the endpoint, blood was
collected to measure indomethacin plasma concentrations and kidneys were collected for
morphological measurement. Stereological techniques were used to measure the glomerular
number and volume in these animals. We observed no significant differences in glomerular
number (p=0.39) and volume (p=0.98) between the different treated groups. In conclusion,
administration of indomethacin, while the process of nephrogenesis is ongoing, does not
significantly reduce/change the glomerular number.
Description
Keywords
Psychology--Physiological
Citation
Baba, H. (2016). Effect of Indomethacin on Kidney Development in Postnatal Period in Rats (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25801