Neurodegeneration: the link between synaptic loss and lysosome function
Abstract
There is a large ongoing international effort to understand the pathogenic cascade underlying neurodegenerative diseases in order to rationally develop therapies. Here we investigate the role of two elements: 1) Changes in BKα channel expression, 2) Changes in lysosomal activity in crude synaptosomes from CSPα KO mice. CSPα is a presynaptic neuroprotective chaperone protein that, when mutated causes synaptic loss and neurodegeneration. BK channel KO, and intraperitoneal injections with chloroquine (lysosomal inhibitor), and Z-Phe-Ala-diazomethylketone which increases lysosomal activity did not halt neurodegeneration or alter the composition of crude synaptosomes. We report that a subset of endo-lysosomal proteins is present in crude synaptosomes.
Description
Keywords
Biology--Cell, Biology--Molecular, Neuroscience, Physiology, Pharmacology
Citation
Donnelier, J. (2016). Neurodegeneration: the link between synaptic loss and lysosome function (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27082