Arabidopsis thaliana Protein Phosphatase 1: A Cell Signalling Engine with Many Keys

Date
2016
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Abstract
Throughout all domains of life the strict regulation of signalling pathways allows for the proper control of cellular processes through the action of protein kinases and protein phosphatases. In the model plant Arabidopsis thaliana, one of the major protein phosphatases, PP1, represents a critical regulator of many signalling pathways. Type one phosphatases (PP1) use regulatory subunits to target dephosphorylation and associate with these regulatory proteins via a highly conserved ‘RVxF’ motif. The regulators of PP1 in Arabidopsis are largely unidentified. Using Arabidopsis stable transformant plant extracts, the regulators of PP1 have been identified. Of the previously known PP1 regulators, GEM is a known root hair differentiation checkpoint protein and contains the PP1 docking motif ‘RVxF’. Using a biophysical approach, I have validated the PP1:GEM interaction through the ‘RVxF’ motif. Finally GEM contains a GRAM domain and the analysis of phosphotidyl-inositol lipid binding to this domain was examined.
Description
Keywords
Biochemistry
Citation
Sieben, N. A. (2016). Arabidopsis thaliana Protein Phosphatase 1: A Cell Signalling Engine with Many Keys (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/24989