The In Vitro Development of a Metabolomic Test for Response to Antineoplastic Drugs

Date
2017
Journal Title
Journal ISSN
Volume Title
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Abstract
Background: Only a fraction of patients benefit from any given chemotherapeutic. An assay that enables early identification of individuals who are/are not benefiting from a drug would be useful. We postulate that a response to systemic therapy is associated with characteristic changes in extracellular metabolites. Methods: Multiple tumor cell lines were exposed to a variety of chemotherapeutics. Supernatants collected at baseline and at 72 hours were analyzed by gas chromatography-mass spectrometry to identify metabolomic changes. An MTT assay was used to quantify growth inhibition. Results: We identified 23 candidate metabolites that change with response to therapy, independent of cell and drug type. This model had a sensitivity and specificity of 0.714 and 0.813 respectively in an ROC analysis. Conclusions: The candidate metabolites identified will be assessed in clinical samples from patients treated with systemic therapy. Identification of response-related changes in the circulating metabolome may represent a novel means of detecting response to chemotherapy.
Description
Keywords
Medicine and Surgery, Oncology
Citation
Amin, S. (2017). The In Vitro Development of a Metabolomic Test for Response to Antineoplastic Drugs (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28224