This thesis examines the use of whole slide imaging (WSI) analysis and staining techniques to maximize the amount of data from an entire glass slide. Although WSI is used in research, data are primarily from specimens obtained from biopsy rather than full thickness sections. To date the use of WSI has not been used to assess the histology of blood vessels. WSI is a method for quantifying components of tunica media and was found to be consistent with the qualitative descriptions in the literature. This data concordance was then applied to compare non-diseased aorta with Type A dissecting aorta. The results demonstrated differences in the comparison between the two vessels. Models derived from pixel-level informatics have the potential to be used for histopathological assessment of vascular wall content.