Motor cortex excitability in vitro: Modulation by cannabinoid 1 receptors and stress
Date
2013-09-13
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Abstract
An investigation was conducted into the activation mode of layer V pyramidal neurons of
the motor cortex in response to intracortical microstimulation (ICMS) used to derive
motor maps. An experimental preparation that combines ICMS and patch clamp singlecell
electrophysiology was used. Bath application of glutamate (AMPA and NMDA) and
GABAA receptor antagonists caused a significant reduction in spiking responses,
suggesting a mainly synaptic mode of activation. These findings validate this preparation
termed slice-ICMS in further examinations of neurotransmitter systems in motor map
expression. In a second study, an investigation into the role of the cannabinoid receptor 1
in the excitability of motor cortex layer V pyramidal neurons was conducted.
Endocannabinoids (eCBs) are ubiquitous retrograde messengers that inhibit
neurotransmitter release in the central nervous system. CB1 receptor activation decreased
excitability in layer V pyramidal neurons measured as a decrease in spiking responses to
ICMS. This effect was compromised when rats were repeatedly stressed, suggesting
downregulation of the CB1 receptor with stress in the motor cortex.
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Neuroscience
Citation
Hussin, A. T. (2013). Motor cortex excitability in vitro: Modulation by cannabinoid 1 receptors and stress (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27015