A Biophysical Characterization of Histone detived Antimicrobial Peptides
Date
2013-09-23
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Histones have been recently shown to be integral to the response of the body to invading pathogens. As such, prior work on this project identified segments of several histones or fragments thereof as potential AMPs. This project comprises a continued biophysical characterization of these selected segments of histone. The project investigates the first generation of AMPs with monolayer models and Brewster angle microscopy to screen for the lipids which are most important in AMP-lipid interactions. Subsequently new variant peptides were designed from one parent peptide (H3) and studied through the use of several thermodynamic and spectroscopic techniques. The variant peptides were shown to be more potent than the parent peptide in terms of microbicidal activity, but were not as selective for bacterial membranes. Therefore, further studies are needed to determine the therapeutic value of the variants.
Description
Keywords
General, General, General, General, General, General, General, General, General, General, General, General, General, General, General, General, General
Citation
Berezowski, N. S. (2013). A Biophysical Characterization of Histone detived Antimicrobial Peptides (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/24634