Multilocus sequence typing of historical burkholderia pseudomallei isolates
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2007
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Abstract
A collection of 207 historically relevant Burkholderia pseudomallei isolates was analyzed by multilocus sequence typing. The collection contains environmental isolates from a geographical survey of B. pseudomallei in Thailand during 1964 to 1967, clinical isolates from American soldiers serving in Vietnam, and stock cultures and colony variants from the United States Army Medical Research Unit (Malaysia), the Walter Reed Army Institute for Research, and the Pasteur Institute (Vietnam). The collection was resolved into 80 sequence types, 56 were novel. Differences in colony morphology among reference isolates did not affect ST assignment and the first environmental isolates sharing STIO with B. pseudomallei K96243 were reported. eBURST analysis of the historical collection demonstrated an ancestral nature of the isolates when compared with all isolates catalogued in the B. pseudomallei MLST database. Australian ST84 was found to be characteristic of B. pseudomallei isolates from Southeast Asia and an epidemiologically significant phylogenetic relationship was discovered between Thai and Australian environmental isolates through the commonality of ST60, which has important implications for future studies in melioidosis epidemiology.
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Bibliography: p. 104-113
Some pages are in colour.
Some pages are in colour.
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McCombie, R. L. (2007). Multilocus sequence typing of historical burkholderia pseudomallei isolates (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/1251