Characterization of plagl1 transcriptome in retinal development
Date
2012
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Abstract
Plagll is a maternally imprinted tumor suppressor gene that encodes a zmc finger transcription factor. Plagll is a key regulator of cell number control and cellular migration in the developing retina. Our previous study showed that deletion of Plagll results in increased proliferation and reduced apoptosis at postnatal stages of retinal development, leading to formation of an ectopic cell layer. To identify transcriptional targets that work downstream of Plagll, we performed a microarray analysis ofE18.5- 4 day in vitro (DIV) wild type and Plagll knockout retinal explants. Ingenuity analysis of the microarray data suggested that several genes involved in cellular functions such as gene expression, cell death, cell cycle, cell growth and proliferation, and movement were differentially expressed in Plagll mutant retinas. Quantification of differential expression of a subset of these genes by quantitative real time PCR identified a few putative targets of Plagll and three of them, Cdknl a, Neurog2 and Ndn, also came differentially expressed with Plagll gain of function study in P19 cells in a direction opposite to that of loss of function study. Moreover, expression analysis by RNA in situ hybridization revealed similar expression patterns as that of Plagll for some of the differentially expressed genes suggesting that they may be downstream targets of Plagll. This work helped to gain some insight into the network of genes that may function downstream of Plagll and help it in executing its regulatory role during retinal development.
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Bibliography: p. 186-210
Many pages are in colour.
Includes copy of animal protocol approval. Original copy with original Partial Copyright Licence.
Many pages are in colour.
Includes copy of animal protocol approval. Original copy with original Partial Copyright Licence.
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Citation
Kaushik, G. (2012). Characterization of plagl1 transcriptome in retinal development (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4770