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Plant Defense Responses in Opium Poppy Cell Cultures Revealed by Liquid Chromatography-Tandem Mass Spectrometry Proteomics

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Author
Zulak, Katherine G.
Khan, Morgan F.
Alcantara, Joenel
Schriemer, David
Facchini, Peter J.
Accessioned
2017-08-17T20:56:57Z
Available
2017-08-17T20:56:57Z
Issued
2008-08-05
Subject
Alkaloids
Benzophenanthridines
Botrytis
Cell Culture Techniques
Chromatography, Liquid
DNA, Complementary
Electrophoresis, Gel, Two-Dimensional
Enzyme Induction
Expressed Sequence Tags
Gene Expression Regulation, Plant
Isoquinolines
Molecular Sequence Data
Papaver
Plant Proteins
Proteomics
RNA, Messenger
Tandem Mass Spectrometry
Papaver somniferum
Type
journal article
Metadata
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Abstract
Opium poppy (Papaver somniferum) produces a diverse array of bioactive benzylisoquinoline alkaloids, including the narcotic analgesic morphine and the antimicrobial agent sanguinarine. In contrast to the plant, cell cultures of opium poppy do not accumulate alkaloids constitutively but produce sanguinarine in response to treatment with certain fungal-derived elicitors. The induction of sanguinarine biosynthesis provides a model platform to characterize the regulation of benzylisoquinoline alkaloid pathways and other defense responses. Proteome analysis of elicitor-treated opium poppy cell cultures by two-dimensional denaturing-polyacrylamide gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry facilitated the identification of 219 of 340 protein spots based on peptide fragment fingerprint searches of a combination of databases. Of the 219 hits, 129 were identified through pre-existing plant proteome databases, 63 were identified by matching predicted translation products in opium poppy-expressed sequence tag databases, and the remainder shared evidence from both databases. Metabolic enzymes represented the largest category of proteins and included S-adenosylmethionine synthetase, several glycolytic, and a nearly complete set of tricarboxylic acid cycle enzymes, one alkaloid, and several other secondary metabolic enzymes. The abundance of chaperones, heat shock proteins, protein degradation factors, and pathogenesis-related proteins provided a comprehensive proteomics view on the coordination of plant defense responses. Qualitative comparison of protein abundance in control and elicitor-treated cell cultures allowed the separation of induced and constitutive or suppressed proteins. DNA microarrays were used to corroborate increases in protein abundance with a corresponding induction in cognate transcript levels.
Grantingagency
Natural Sciences and Engineering Research Council of Canada Discovery and Strategic Grants (to P. J. F.).
Refereed
Yes
Sponsorship
This work was supported by funds provided by Natural Sciences and Engineering Research Council of Canada Discovery and Strategic Grants (to P. J. F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Citation
Zulak, K. G., Khan, M. F., Alcantara, J., Schriemer, D. C., & Facchini, P. J. (2009). Plant defense responses in opium poppy cell cultures revealed by liquid chromatography-tandem mass spectrometry proteomics. Molecular & Cellular Proteomics, 8(1), 86-98. doi:10.1074/mcp.M800211-MCP200
Department
Department of Biological Sciences
Faculty
Science
Institution
University of Calgary
Url
http://www.mcponline.org
Publisher
Molecular & Cellular Proteomics
Doi
http://dx.doi.org/10.1074/mcp.M800211-MCP200
http://dx.doi.org/10.11575/PRISM/33813
Uri
http://hdl.handle.net/1880/52193
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