Phenotypic and Functional Analysis of Regulatory T Cells during Heligmosomoides polygyrus infection

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dc.contributor.advisorFinney, Constance
dc.contributor.authorBowron, Joel
dc.contributor.committeememberMcKay, Derek
dc.contributor.committeememberPeters, Nathan
dc.date2018-06
dc.date.accessioned2018-01-19T00:57:14Z
dc.date.available2018-01-19T00:57:14Z
dc.date.issued2018-01-04
dc.description.abstractHelminths are known to stimulate regulatory T cells, which have been shown to downregulate inflammatory as well as allergic conditions such as Crohn’s Disease, Ulcerative Colitis and allergic asthma respectively. However, the differences in the Tregs generated during helminth infection compared to steady state natural T regs have yet to be fully elucidated. Classically, in mice, Tregs are defined as CD4+CD25+ T cells. We have demonstrated that, unlike in BALB/c mice, in C57BL/6 animals, infection with the gastrointestinal nematode Heligmosomoides polygyrus does not result in increased CD4+CD25+-mediated suppressive activity. Using two different infection protocols, we characterized the CD4+CD25+ Treg-mediated response to acute and chronic infection from two different gut lymphoid organs, the Peyers patches and mesenteric lymph nodes. Unexpectedly, we found Tregs isolated from infected animals to be equally or less suppressive compared to those from naïve controls. However, we found no difference in the expression of Helios or CTLA-4, markers that play a role in control of the regulatory T cell phenotype and suppress T cell responses, respectively. These data indicate that manipulation of Tregs by helminths are more complex than previously thought and require more investigation into their suppressive profiles.en_US
dc.identifier.citationBowron, J. C. (2018). Phenotypic and Functional Analysis of Regulatory T Cells during Heligmosomoides polygyrus infection (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/5363
dc.identifier.urihttp://hdl.handle.net/1880/106282
dc.language.isoenen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subject.classificationParasitologyen_US
dc.subject.classificationImmunologyen_US
dc.titlePhenotypic and Functional Analysis of Regulatory T Cells during Heligmosomoides polygyrus infectionen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrue
ucalgary.thesis.checklistI confirm that I have submitted all of the required forms to Faculty of Graduate Studies.en_US
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