Genetic regulation of chemotaxis and motility in Rhizobium leguminosarum

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dc.contributor.advisorHynes, Michael F.
dc.contributor.authorDel Bel, Kathryn L.
dc.date.accessioned2005-08-16T16:55:16Z
dc.date.available2005-08-16T16:55:16Z
dc.date.issued2004
dc.descriptionBibliography: p. 134-142en
dc.descriptionSome pages are in colour.en
dc.description.abstractRhizobium leguminosarum, a nitrogen-fixing root nodule bacterium, has over 20 genes coding MCP-type chemoreceptors, which could playa role in locating host legumes by chemotaxis. Transcriptional gusA fusions were made with the mcpC and mcpD genes, and were used to confirm that the mcp genes are down-regulated in the nodule. Transposon mutagenesis was completed with a mcpC fusion strain to identify possible genes which regulate mcp expression. A free-living mcp regulatory mutant was identified as a bacA mutation, while two mutants which showed less down-regulation in nodules than the wild-type were identified as hutC and a ribitiol transporter mutant. Changes in expression in these mutants in planta were confirmed by examining nodules histologically. The flagellin proteins from a R. leguminosarum strain were isolated and used to produce an anti-flagellar antibody. Immunoblots employing this antibody confirmed that flagella, like MCPs, are down-regulated in nodule bacteria.en
dc.format.extentxii, 145 leaves : ill. ; 30 cm.en
dc.identifier.citationDel Bel, K. L. (2004). Genetic regulation of chemotaxis and motility in Rhizobium leguminosarum (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/16625en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/16625
dc.identifier.isbn0612976408en
dc.identifier.lccAC1 .T484 2004 D455en
dc.identifier.urihttp://hdl.handle.net/1880/41448
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleGenetic regulation of chemotaxis and motility in Rhizobium leguminosarum
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1497 520492014
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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