Identifying physiological substrates of DNA-dependent protein kinase (DNA-PK)
Date
2004
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The DNA-dependent protein kinase (DNA-PK) is required for the repair of DNA double strand breaks (DSB) in human cells. DNA-PK is a serine/threonine protein kinase composed of a 469 kDa catalytic subunit (DNA-PKcs) and a DNA targeting subunit, Ku.
Previous studies have demonstrated a requirement of the protein kinase activity of DNA-PK during non homologous end joining-mediated repair of DNA DSBs and during variable (diversity) joining [V(D)J] recombination. However, its physiological substrates are not well defined.
Here we have utilized a broad-based proteomics approach to attempt to identify DNA-PK-dependent phosphorylation events. Utilizing a more targeted approach involving immunoprecipitation of DNA-PKcs, an ionizing radiation-dependent interaction between DNA-PKcs and p53 transcription factor was identified in human lymphoblastoid cells.
Description
Bibliography: p. 131-150
Keywords
Citation
Bader, M. (2004). Identifying physiological substrates of DNA-dependent protein kinase (DNA-PK) (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/13641