The analyses presented in this dissertation reconceptualized the philosophical speculations that underpinned the epistemological foundations of Margaret Whitehead’s Physical Literacy paradigm with attention to scientific claims of evidence. Establishing a scientific foundation for physical literacy as a holistic, immersive paradigm led to the notion that interactive experiences are laboratory exemplars for testing the motivation of Physical Literacy’s holistic experiences. This led to a randomized, comparative trial (RCT) of two cycling interfaces - 2D monitor and 360° headset – that demonstrated, for both conditions, motivation to engage in physical activity while exergaming increased equally. Experiences associated with 2D monitors and 360° VR headsets with interactive cycling and gaming illustrated a safe and enjoyable way to engage seniors in mitigating sedentary behavior while actively engaging in physical activity. This has important implications for the design and development of exergaming systems. The results of the RCT study found that interactive exergaming while cycling with immersive interfaces led to increased enjoyment, was encouraging, and gave rise to an exploration that has potential to foster lifelong engagement in physical activity. A Delphi Method enabled experts to generate and discuss recommendations for healthier lifespans by managing physical and sedentary behaviors across the lifespan.