Immunolocalization and lc-ms/ms proteomics reveal in interplay between sieve elements and laticifers in the biosynthesis of morphine in opium poppy
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2012
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Abstract
Opium poppy produces a group of natural products called benzylisoquinoline alkaloids (BIAs) such as morphine and codeine. BIAs have been shown to be synthesized in the phloem specifically in sieve elements, and accumulate in the latex, which is the cytoplasm of specialized secretory cells called laticifers. This project aimed to identify the cell types involved morphine biosynthesis via immunolocalization of six biosynthetic enzymes and LC-MS/MS proteomics analysis on latex and whole stem proteins. This resulted in the localization of codeine 0-demethylase (CODM), the last enzyme involved in morphine biosynthesis in laticifers, different from the other biosynthetic enzymes. This indicates a biosynthetic role for the laticifers. The proteomes showed the presence of morphine biosynthetic enzymes and other BIA biosynthetic enzymes. A new model is proposed where both cell types function in synthesizing morphine, with the penultimate steps occurring predominantly in laticifers. It also suggests the need for intercellular transport of alkaloid.
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Bibliography: p. 87-99
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Onoyovwi, A. (2012). Immunolocalization and lc-ms/ms proteomics reveal in interplay between sieve elements and laticifers in the biosynthesis of morphine in opium poppy (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4926