Discerning Alveolar Macrophage Ontogeny in Mice

Zhang, Wen Xuan

Discerning Alveolar Macrophage Ontogeny in Mice

dc.contributor.advisor	Kubes, Paul
dc.contributor.author	Zhang, Wen Xuan
dc.contributor.committeemember	Jenne, Craig
dc.contributor.committeemember	Kelly, Margaret
dc.date	2023-11
dc.date.accessioned	2023-06-30T21:15:36Z
dc.date.available	2023-06-30T21:15:36Z
dc.date.issued	2023-06
dc.description.abstract	The adaptive and innate immunity are the two branches of the immune system our body uses to defend against invading microbes. After each encounter with a pathogen, the adaptive immune system develops a memory to prevent an infection with the same microbe. This ability to remember has recently also been shown in the innate immune system and was termed “trained immunity”, describing the ability to generate a stronger response towards a broad range of pathogens despite infection with just a single pathogen. The lung is an organ directly exposed to the microbes in the outside world, making it a likely site for immune cells to develop trained immunity. The predominant innate immune cells that reside in the lungs are the alveolar macrophages. These cells encounter countless pathogens every day and therefore are a likely cell in which to study trained immunity. Our lab recently figured out how to watch the macrophages crawl inside the lungs of living animals and this study examined how these macrophages behaved under different conditions (Neupane et al, 2020). However, these macrophages only live for a few months, so cells from the bone marrow constantly replace the macrophages, meaning that there are always two types of macrophages present in the lung. However, without an effective labelling tool, it was previously impossible to distinguish between the two macrophage populations, making their differences in behavior and function unknown. This study utilized a unique model to look at the two different macrophages and visualize these cells inside a living mouse to look for behavioural similarities and differences. Our findings determined the behavioral and functional similarities of these macrophages at homeostasis, their responses against the influenza virus, and provided support for both populations to be trained against influenza.
dc.identifier.citation	Zhang, W. X. (2023). Discerning alveolar macrophage ontogeny in mice (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.uri	https://hdl.handle.net/1880/116685
dc.identifier.uri	https://dx.doi.org/10.11575/PRISM/41527
dc.language.iso	en
dc.publisher.faculty	Graduate Studies
dc.publisher.institution	University of Calgary
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject	Immunology
dc.subject	lung
dc.subject	virus
dc.subject	macrophage
dc.subject	imaging
dc.subject	intravital
dc.subject	monocyte
dc.subject	confocal
dc.subject.classification	Education--Health
dc.subject.classification	Immunology
dc.title	Discerning Alveolar Macrophage Ontogeny in Mice
dc.type	master thesis
thesis.degree.discipline	Medicine – Immunology
thesis.degree.grantor	University of Calgary
thesis.degree.name	Master of Science (MSc)
ucalgary.thesis.accesssetbystudent	I do not require a thesis withhold – my thesis will have open access and can be viewed and downloaded publicly as soon as possible.