The effect of shortening on crossbridge dynamics in cardiac muscle

dc.contributor.advisorter Keurs, Henk E.D.J.
dc.contributor.authorDeis, Nathan Patrick
dc.date.accessioned2005-08-19T20:40:04Z
dc.date.available2005-08-19T20:40:04Z
dc.date.issued2004
dc.descriptionBibliography: p. 96-105en
dc.description.abstractLandesberg and Sideman have proposed a model for cross-bridge cycling where the rate constant for crossbridge weakening, G, is a function of the sarcomere shortening velocity such that G = G0 + G1 • V , where G0 is the rate constant in the isometric state and G\ is the mechanical feedback. The effect of myosin heavy chain isoform on G1 is unknown. Methods: Rats were made to express either pure α-MHC, by treatment with thyroid hormone (T3), or pure ß-MHC, by treatment with PTU. Force-velocity curves were obtained from cardiac trabeculae (Ca2+ = 1.5 mM, 25°C). To determine G1, ~FN the instantaneous force difference between a shortening and isometric contraction was measured. Results: The sarcomere shortening velocity of T3 rats were twice that of PTU treated rats at all levels of relative load (VMAX= 15.99 µm/s - T3, 8.95 µm/s - PTU). There was no difference in the curvature of the force-velocity relationship between the two groups. G1 as measured from slow ( ≤ 4 µm/s) releases was 4.76 /µm (STD DEV = 1.43) for T3 rats and 4.88 /µm (STD DEV = 1.29) for PTU rats.en
dc.format.extentx, 123 leaves : ill. ; 30 cm.en
dc.identifier.citationDeis, N. P. (2004). The effect of shortening on crossbridge dynamics in cardiac muscle (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/22878en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/22878
dc.identifier.isbn0612976394en
dc.identifier.lccAC1 .T484 2004 D45en
dc.identifier.urihttp://hdl.handle.net/1880/42317
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleThe effect of shortening on crossbridge dynamics in cardiac muscle
dc.typemaster thesis
thesis.degree.disciplineCardiovascular & Respiratory Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1496 520492013
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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