A Role for Exchange of Extracellular Vesicles in Porcine Spermatogonial Co-Culture

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dc.contributor.advisorDobrinski, Ina
dc.contributor.authorThiageswaran, Shiama
dc.contributor.committeememberKlein, Claudia
dc.contributor.committeememberRancourt, Derrick
dc.contributor.committeememberGilch, Sabine
dc.date2022-11
dc.date.accessioned2022-08-02T21:18:03Z
dc.date.available2022-08-02T21:18:03Z
dc.date.issued2022-07-08
dc.description.abstractSpermatogonial stem cells (SSCs) provide the basis for lifelong male fertility through self-renewal and differentiation. Prepubertal male cancer patients may be rendered infertile by gonadotoxic chemotherapy and, unlike sexually mature men, cannot store sperm. Testicular biopsies taken prior to treatment may be used to restore fertility in adulthood. Testicular SSC populations are limited, necessitating in vitro culture systems to increase the numbers of SSCs available for downstream applications. Using the pig as a non-rodent model, we developed spermatogonial culture systems to expand spermatogonia from 1- and 8-week-old porcine testes, comparing feeder layers consisting of populations enriched for Sertoli cells, peritubular myoid cells (PMCs), pig fetal fibroblasts (PFFs), and testicular endothelial cells (TECs). As previously developed porcine spermatogonial culture systems relied exclusively on Sertoli cell feeder layers, we explored whether constituent cells of the SSC niche, such as PMCs and TECs, or fibroblastic cells like PFFs, may also support SSC expansion. Spermatogonia co-cultured with PMCs and PFFs had comparable rates of proliferation and apoptosis to spermatogonia co-cultured with Sertoli cells. To elucidate the mechanism behind the beneficial nature of feeder layers, we investigated the role of extracellular vesicles in the dynamic crosstalk between spermatogonia and feeder cells. Sertoli cell-released exosomes were found to be taken up by spermatogonia, and the inhibition of exosomal release reduced spermatogonial proliferation.en_US
dc.identifier.citationThiageswaran, S. (2022). A Role for Exchange of Extracellular Vesicles in Porcine Spermatogonial Co-Culture (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.urihttp://hdl.handle.net/1880/114906
dc.identifier.urihttps://dx.doi.org/10.11575/PRISM/39955
dc.language.isoengen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectspermatogonial stem cellsen_US
dc.subjectspermatogoniaen_US
dc.subjectco-culturesen_US
dc.subjectextracellular vesiclesen_US
dc.subjectpigen_US
dc.subject.classificationBiologyen_US
dc.subject.classificationBiology--Molecularen_US
dc.subject.classificationBiochemistryen_US
dc.titleA Role for Exchange of Extracellular Vesicles in Porcine Spermatogonial Co-Cultureen_US
dc.typemaster thesisen_US
thesis.degree.disciplineMedicine – Biochemistry and Molecular Biologyen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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