Improving Algae Growth Kinetics in Suspension Bioreactors for the Production of Recombinant Proteins

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atmire.migration.oldid5057
dc.contributor.advisorSen, Arindom
dc.contributor.advisorAlcantara, Joenel
dc.contributor.authorClark, Brendan Robert
dc.contributor.committeememberHollenberg, Morley
dc.contributor.committeememberDe la Hoz Siegler, Hector
dc.contributor.committeememberGates, Ian
dc.contributor.committeememberTay, Andrew
dc.date.accessioned2016-10-11T15:39:57Z
dc.date.available2016-10-11T15:39:57Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractMillions of individuals rely on recombinant proteins such as essential biopharmaceuticals. Recently, genetically engineered microalgae have been identified as a potentially inexpensive and fast growing host organism for recombinant protein production. Using Chlamydomonas reinhardtii, a species of unicellular green microalgae, the goal was to improve algal cell growth kinetics, genetically engineer the cells and develop a bioprocess to analyze recombinant protein production. C. reinhardtii growth kinetics were improved under mixotrophic growth conditions using acetate in small scale 10 mL cultures. This process was scaled-up to 500 mL spinner flask suspension bioreactors and through the use of a fed-batch acetate feeding strategy, cell growth rates and maximum cell concentrations were improved. A genetic construct was designed, manufactured, isolated and used to genetically transform C. reinhardtii. A bioprocess was then developed to isolate and analyze protein production rates from these cells. Results indicated product concentrations of 8.44 mg/L of culture.en_US
dc.identifier.citationClark, B. R. (2016). Improving Algae Growth Kinetics in Suspension Bioreactors for the Production of Recombinant Proteins (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26485en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26485
dc.identifier.urihttp://hdl.handle.net/11023/3422
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectEngineering--Biomedical
dc.subjectEngineering--Chemical
dc.subject.classificationBioprocess Engineeringen_US
dc.subject.classificationBiochemical Engineeringen_US
dc.subject.classificationChlamydomonas reinhardtiien_US
dc.subject.classificationAlgal Biotechnologyen_US
dc.subject.classificationRecombinant Protein Productionen_US
dc.subject.classificationGenetic Engineeringen_US
dc.subject.classificationChemical Engineeringen_US
dc.subject.classificationMicroalgae Cultureen_US
dc.titleImproving Algae Growth Kinetics in Suspension Bioreactors for the Production of Recombinant Proteins
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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