Turner, Raymond JosephMiclat, Jason2018-04-132018-04-132018-04-12Miclat, J. (2018). Calcium Regulation of a Slow AHP in Hippocampal Pyramidal Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/31781http://hdl.handle.net/1880/106493A slow afterhyperpolarization (sAHP) in CA1 hippocampal pyramidal cells is calcium-dependent and involves activation of KCa3.1 and KCNQ potassium channels. Both KCa3.1 and KCNQ channels bind calmodulin (CaM) as a calcium sensor, with an additional role proposed for the protein hippocalcin. We measured the calcium sensitivity of the IsAHP and potassium channel associations with CaM or hipppocalcin. In recordings in hippocampal tissue slices in vitro the KCa3.1-mediated IsAHP steadily increased over 10 min equilibration to 0 µM calcium electrolyte, but decreased upon infusing 1 µM calcium, while the KCNQ-mediated IsAHP responded in an opposite manner. KCa3.1 channels contributed to the IsAHP in rat but not mouse pyramidal cells. Coimmunoprecipitation occurred between KCa3.1 and both CaM and hippocalcin, but only KCNQ and CaM. These data reveal a species-specific expression pattern for two potassium channels and in calcium sensor proteins present to generate the sAHP in the same class of hippocampal neuron.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.slow afterhyperpolarizationhippocampusCA1hippocalcincalmodulinKCa3.1KCNQNeurosciencemaster thesis10.11575/PRISM/31781