Desgagné-Penix, IsabelKhan, Morgan F.Schriemer, David C.Cram, DustinNowak, JacekFacchini, Peter J.2017-06-012017-06-012010-11-18Desgagné-Penix, I., Khan, M. F., Schriemer, D. C., Cram, D., Nowak, J., & Facchini, P. J. (2010). Integration of deep transcriptome and proteome analyses reveals the components of alkaloid metabolism in opium poppy cell cultures. BMC Plant Biology, 10(1), 252-252. doi:10.1186/1471-2229-10-2521471-2229http://hdl.handle.net/1880/52008Papaver somniferum (opium poppy) is the source for several pharmaceutical benzylisoquinoline alkaloids including morphine, the codeine and sanguinarine. In response to treatment with a fungal elicitor, the biosynthesis and accumulation of sanguinarine is induced along with other plant defense responses in opium poppy cell cultures. The transcriptional induction of alkaloid metabolism in cultured cells provides an opportunity to identify components of this process via the integration of deep transcriptome and proteome databases generated using next-generation technologies.enBerberine Bridge EnzymePapaver-SomniferumMolecular-CloningMorphine BiosynthesisDihydrobenzophenanthridine OxidaseNorcoclaurine SynthaseN-MethyltransferaseSuspension CulturesGene TranscriptExpressionjournal article10.1186/1471-2229-10-25210.11575/PRISM/33801