Knight, Cameron G.Klein, C.Wachoski-Dark, Garrett Louis2018-10-092018-10-092018-09-28Wachoski-Dark, G. L. (2018). Investigation into Equine Papillomavirus Type 2 Prevalence and Diagnosis (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/33154http://hdl.handle.net/1880/108814Equine papillomavirus type 2 (EcPV-2) has been consistently associated with most equine genital cancers and a subset of cancers at other locations. The prevalence of EcPV-2 in healthy horses has been estimated to be <20% for DNA presence, and 10-36% for anti-EcPV-2-L1 seroprevalence. However, no study has thus far examined the prevalence of EcPV-2 at the herd-level or over multiple timepoints. This thesis examined EcPV-2 prevalence in a predominantly closed herd with a known EcPV-2 infected horse over three timepoints. We found that DNA prevalence (0-4%) was equivalent to the general horse population, but that seroprevalence (51.5%) was higher. Additionally, no study has examined the distribution of EcPV-2 infection in situ, nor compared such a technique to PCR. We found that RNA ISH concorded with PCR in 80% of cases. We also recapitulated previous results concerning EcPV-2 distribution in situ as well as found EcPV-2 in numerous novel locations.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.EcPV-2equine papillomavirus type 2Veterinary ScienceEpidemiologyPathologyEngineering--Biomedicalmaster thesis10.11575/PRISM/33154