Shi, YanMucsi, Ashley Dawn2017-12-182017-12-182012Mucsi, A. D. (2012). The nature of regulatory t cell interactions with dendritic cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4954http://hdl.handle.net/1880/105955Bibliography: p. 93-103Some pages are in colour.Includes copy of animal protocol approval. Original copy with original Partial Copyright Licence.The immune system has developed several mechanisms responsible for discriminating against self and non-self antigens and preventing autoimmune responses from occurring within the body. Inactivation of auto-reactive lymphocytes and elimination of these cells prevents the generation of autoimmune responses ensuring that tolerance results in the periphery. A specialized subpopulation of CD4+ T cells, regulatory T cells (Tregs ), maintains peripheral self-tolerance through a diverse set of mechanisms. The mechanisms utilized by regulatory T cells are vast; however, the mechanisms by which Tregs modulate their suppressive function are not yet fully understood. Utilizing atomic force microscopy, we demonstrate that IL-2 stimulated Tregs interact intensely with dendritic cells (DC), which is dependent upon the LFA-1/ICAM-1 adhesion molecule. We propose that LF A-1 regulation differs between Tregs and CD4+ CD25- T cells, allowing Tregs to interact intensely with DCs abrogating any interaction with antigen­specific T cells. Our results illustrate how Tregs interact with DCs and its association with LF A-1 avidity regulation.xi, 103 leaves : ill. ; 30 cm.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.master thesis10.11575/PRISM/4954