Wildering, WillemPerry, Alexander2017-08-172017-08-1720172017http://hdl.handle.net/11023/4023Lipid peroxidation is a symptom of aging that is suspected to cause undesirable alterations to membrane properties. These alterations are postulated to induce membranes defects, which negatively affect aspects of cells and hinders their functionality. Neuronal plasma membranes contain constituents that are particularly susceptible to oxidative species; therefore, they are prime targets for lipid peroxidation. Neurons from Lymnaea stagnalis were used in combination with various fluorescent probes to assess membrane hydrophobic barrier function to exclude the entry of ionic molecules through unassisted means. However, instead of exclusively probing passive permeability, it was discovered that various forms of active membrane transport processes in neuronal membranes may also be regulated by redox reactions. Contrary to suggestions from modeling studies, lipid peroxidation appeared to only slightly alter passive membrane permeability, suggesting that biological membranes may contain certain constituents and/or mechanisms that prevents lipid peroxidation from being as detrimental as observed under artificial conditions.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Education--SciencesAnimal PhysiologyBiology--CellNeurosciencePhysiologyZoologyOxidative stressagingLymnaea stagnalisNeuronCell cultureABC transporterMulti-drug resistance-associated proteinROSPlasma membraneEndocytosisLysosomesPropidium iodideCarboxyfluoresceinProbenecidFluorescein diacetatemaster thesis10.11575/PRISM/28110