Dobrinski, InaBurrell, Kyle2018-01-192018-01-192017-12-21Burrell, K. (2017). Stirred Suspension Bioreactor Culture for the Expansion of Porcine Induced Pluripotent Stem Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.http://hdl.handle.net/1880/106290Induced pluripotent stem cells are an attractive cell source for regenerative medicine and the development of therapies, as they can differentiate into any cell type in the body and can proliferate indefinitely under defined conditions. Traditional cell expansion is limited due to the use of adherent culture, making it difficult to obtain adequate cell numbers for research. It has been previously shown that stirred suspension bioreactors can alleviate this issue when culturing mouse and human stem cells. This study outlines the use of stirred suspension bioreactors as an alternative culture method for the expansion of porcine induced pluripotent stem cells, comparing it to traditional static culture. As pigs are more anatomically and physiologically similar to humans than rodents are, this culture method would provide an adequate cell source for future research applications involving porcine induced pluripotent stem cells.enUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Education--SciencesBiology--CellMicrobiologymaster thesis10.11575/PRISM/5371