Hynes, Michael F.Del Bel, Kathryn L.2005-08-162005-08-162004Del Bel, K. L. (2004). Genetic regulation of chemotaxis and motility in Rhizobium leguminosarum (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/166250612976408http://hdl.handle.net/1880/41448Bibliography: p. 134-142Some pages are in colour.Rhizobium leguminosarum, a nitrogen-fixing root nodule bacterium, has over 20 genes coding MCP-type chemoreceptors, which could playa role in locating host legumes by chemotaxis. Transcriptional gusA fusions were made with the mcpC and mcpD genes, and were used to confirm that the mcp genes are down-regulated in the nodule. Transposon mutagenesis was completed with a mcpC fusion strain to identify possible genes which regulate mcp expression. A free-living mcp regulatory mutant was identified as a bacA mutation, while two mutants which showed less down-regulation in nodules than the wild-type were identified as hutC and a ribitiol transporter mutant. Changes in expression in these mutants in planta were confirmed by examining nodules histologically. The flagellin proteins from a R. leguminosarum strain were isolated and used to produce an anti-flagellar antibody. Immunoblots employing this antibody confirmed that flagella, like MCPs, are down-regulated in nodule bacteria.xii, 145 leaves : ill. ; 30 cm.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.master thesis10.11575/PRISM/16625AC1 .T484 2004 D455