Nguyen, Minh DangParsons, Kari2014-05-212015-11-182014-05-212014http://hdl.handle.net/11023/1540p600 is a large multifunctional protein with important roles in protein degradation, cell adhesion, migration and survival. In the central nervous system (CNS), p600 impacts neurogenesis, neuronal migration, and neuronal survival through interactions with microtubules and calmodulin. The C-terminus of p600 containing the microtubule-binding domains interacts with Ndel1 in vitro and in cultured cells. In the developing neocortex, Ndel1, together with Lis1 and the motor Dynein, regulates mitotic spindle orientation in apical progenitors in a manner reminiscent of p600. My thesis investigates the significance of the p600/Ndel1 interaction. I demonstrate an in vivo association between Ndel1 and p600 in brain tissue. In mitotic cells, I found that overexpression of a NDEL1-binding p600 fragment alters NDEL1's subcellular distribution as well as NDEL1/LIS1 ratios critical in Dynein localization. Finally, I report p600 expression in apical progenitors. My work provides mechanistic insight into the cellular roles of p600, including the function of p600 in neurogenesis.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Neurosciencep600UBR4ZUBR1master thesis10.11575/PRISM/27132