Lee, JihyunKim, KeekyoungShin, Joonhwan2024-05-212024-05-212024-05-14Shin, J. (2024). Development of a multi-axis robotic embedded bioprinting platform and its process chain (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.https://hdl.handle.net/1880/118798https://doi.org/10.11575/PRISM/463953D bioprinting is a tissue engineering technology, and it has successfully developed simple tissues. However, the current bioprinting methods that rely on layer-based cartesian mechanisms face significant challenges in creating complex and vascularized tissues necessary for developing fully functional tissues. This limitation highlights the need for innovative approaches that enhance bioprinting's flexibility and precision. This thesis presents the development and application of a multi-axis robotic bioprinting platform and its process chain that overcomes traditional constraints and enables the fabrication of complex 3D scaffolds from all directions within the expanded workspace. This research involves embedded bioprinting, an extrusion-based method that can bioprint soft and low-viscosity bioinks while maintaining desired printing fidelity using a viscoplastic suspension bath. The multi-axis robotic bioprinting platform, equipped with a 6-degree-of-freedom robotic arm and a pneumatic extrusion system, integrates computer-aided design (CAD) extraction, computer-aided manufacturing (CAM) slicing, robot simulation, script adjustment, and robot control. This process chain facilitates the seamless transition from digital models to physical bioprinted constructs. Two case studies experimentally validate the platform's superiority over traditional bioprinting techniques. The first focuses on freeform surface bioprinting, highlighting the system's adaptability in reproducing intricate tissue contours. The second explores the fabrication of a hollow tubular structure essential for engineering complex vascular networks. In summary, this thesis contributes to developing the technology and processes necessary to standardize in situ/in vivo bioprinting for fabricating artificial tissues and organs directly on damaged sites.enUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.Robotic bioprinting platformRobotic bioprinting process chain3D bioprintingEmbedded bioprintingSix degree-of-freedom robotEngineering--MechanicalEngineering--BiomedicalRoboticsmaster thesis