Buret, AndreDuquette, Stephanie2013-09-062013-11-122013-09-062013Duquette, S. (2013). Anti-inflammatory Properties of Retinoids and Retinoid Derivatives in Innate Immune Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/24936http://hdl.handle.net/11023/926Clearance of apoptotic neutrophils following infection is critical for the resolution of inflammation. Despite demonstrating immunomodulatory properties, the effects of retinoids in neutrophils in the context of an inflammatory response remain unknown. The objective of this project was to evaluate the immunomodulatory properties of retinoic acid (RA) and oxidatively transformed carotene-β (OxC-β) in a model of Mannheimia haemolytica-induced bovine respiratory disease, which is characterized by severe inflammation. Results in vitro and in vivo demonstrate that both RA and OxC-β induce apoptosis in bovine neutrophils and that this phenomenon promotes efferocytosis in bovine macrophages. These effects occur in the absence of antimicrobial properties and any alteration in neutrophil function. The induction of neutrophil apoptosis and their subsequent phagocytic clearance by macrophages are key mechanisms that promote the resolution of inflammation following infection. As such, RA and OxC-β may represent novel nutraceuticals that confer anti-inflammatory benefits following infection.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.CellRetinoic acidOxidatively transformed carotene-betaNeutrophilApoptosisResolution of inflammationmaster thesis10.11575/PRISM/24936