Lees-Miller, Susan P.Bader, Mohamed2005-08-162005-08-162004Bader, M. (2004). Identifying physiological substrates of DNA-dependent protein kinase (DNA-PK) (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/136410612976289http://hdl.handle.net/1880/41739Bibliography: p. 131-150The DNA-dependent protein kinase (DNA-PK) is required for the repair of DNA double strand breaks (DSB) in human cells. DNA-PK is a serine/threonine protein kinase composed of a 469 kDa catalytic subunit (DNA-PKcs) and a DNA targeting subunit, Ku. Previous studies have demonstrated a requirement of the protein kinase activity of DNA-PK during non homologous end joining-mediated repair of DNA DSBs and during variable (diversity) joining [V(D)J] recombination. However, its physiological substrates are not well defined. Here we have utilized a broad-based proteomics approach to attempt to identify DNA-PK-dependent phosphorylation events. Utilizing a more targeted approach involving immunoprecipitation of DNA-PKcs, an ionizing radiation-dependent interaction between DNA-PKcs and p53 transcription factor was identified in human lymphoblastoid cells.xiv, 152 leaves : ill. ; 30 cm.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.master thesis10.11575/PRISM/13641AC1 .T484 2004 B335