Robbins, StephenBox, Alan Kirby2005-08-162005-08-162004Box, A. K. (2004). Differential sorting of ephrin ligands in a polarized cell line (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/222450612975266http://hdl.handle.net/1880/42188Bibliography: p. 137-169Some pages are in colour.The Eph family of receptor is the largest family of receptor tyrosine kinases known. The ephrins are the membrane tethered ligands for this system. The ephrins are divided into two classes: the glycosylphosphatidylinositol anchored A-class ephrins, and the transmembrane B-class ephrins. Both classes of ephrins are associated with lipid rafts. The ephrins were expressed in MDCK cells, a polarized epithelial cell line that uses lipid rafts in sorting. Ephrin-A5 is apically sorted while ephrin-Bl is basolaterally sorted in this system as judged by immunofluorescence and Western blotting. The C-terminus of ephrin-Bl is not involved in its sorting in MDCK cells. The two classes of ephrins are differentially sensitive to Triton X-IOO and Brij 58, suggesting that they may be localized to different lipid rafts. The presence of ephrins in MDCK cells alters the actin cytoskeleton, which may explain the enhanced response to HGF/SF in this system.xiv, 169 leaves : ill. ; 30 cm.engUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.master thesis10.11575/PRISM/22245AC1 .T484 2004 B69