Jung, SunghoonPanchalingam, Krishna M.Rosenberg, LawrenceBehie, Leo A.2018-09-272018-09-272012-05-07Sunghoon Jung, Krishna M. Panchalingam, Lawrence Rosenberg, and Leo A. Behie, “Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media,” Stem Cells International, vol. 2012, Article ID 123030, 21 pages, 2012. doi:10.1155/2012/123030http://hdl.handle.net/1880/108339https://doi.org/10.11575/PRISM/44764Human mesenchymal stem cells (hMSCs) are presently being evaluated for their therapeutic potential in clinical studies to treat various diseases, disorders, and injuries. To date, early-phase studies have indicated that the use of both autologous and allogeneic hMSCs appear to be safe; however, efficacy has not been demonstrated in recent late-stage clinical trials. Optimized cell bioprocessing protocols may enhance the efficacy as well as safety of hMSC therapeutics. Classical media used for generating hMSCs are typically supplemented with ill-defined supplements such as fetal bovine serum (FBS) or human-sourced alternatives. Ideally, culture media are desired to have well-defined serum-free formulations that support the efficient production of hMSCs while maintaining their therapeutic and differentiation capacity. Towards this objective, we review here current cell culture media for hMSCs and discuss medium development strategies.Journal Article2018-09-27enCopyright © 2012 Sunghoon Jung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.https://doi.org/10.1155/2012/123030